Roles of plant growth substance in callus induction of Achyranthes bidentata

H. Duan, Wei Ding, Jianying Song, Jiaming Xu, Huina Wang, Yanqiu Zhu, Wenxiao Liu, Yanqing Zhou
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引用次数: 1

Abstract

In this research, callus from leaves, petioles and stems of Achyranthes bidentata was evidently initiated by plant growth substance, in which 2,4-dichlorophenoxyacetic acid (2,4-D) was very important to callus induction, but effects of other plant growth substances were various, and the optimum combination of plant growth substances for callus induction from leaves, petioles and stems was respectively obtained. Compared with callus induction from leaves and petioles, callus induction from stems was easier, and the higher induction rate and bigger mass of callus from stems were obtained. This study showed that the dedifferentiation capacity of various explants from Achyranthes bidentata was obviously different, and effects of plant growth substance on callus induction from various explants of Achyranthes bidentata were significantly diverse.
植物生长物质在牛膝愈伤组织诱导中的作用
本研究中,牛膝的叶片、叶柄和茎的愈伤组织均明显受到植物生长物质的诱导,其中2,4-二氯苯氧乙酸(2,4- d)对愈伤组织的诱导作用非常重要,但其他植物生长物质的诱导作用各不相同,得出了植物生长物质对叶片、叶柄和茎的愈伤组织诱导的最佳组合。与叶片和叶柄诱导愈伤组织相比,茎诱导愈伤组织更容易,诱导率更高,愈伤组织质量更大。本研究表明,牛膝不同外植体的脱分化能力存在明显差异,植物生长物质对牛膝不同外植体愈伤组织诱导的影响也存在显著差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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