PERKECAMBAHAN DAN PREDIKSI MASA HIDUP TIGA JENIS Dendrobium MENGGUNAKAN EMPAT MACAM FORMULASI MEDIA

Abstract

Dendrobium is an important genus in Orchidaceae because it is widely used in crossbreeding and cut flowers. Seed is a valuable material for propagation as well as useful for maintaining genetic diversity. Seed banks play an important role in orchid conservation. This study aimed to find out the viability of Dendrobium macrophyllum, D. discolor, and D. crumenatum seeds, after being stored for 10 years, predicting the lifespan of seed, and knowing the appropriate germination medium. The method of testing seed viability is directly by germinating the seeds orchids on 4 culture media and calculate the percentage of seed germination. Seeds are stored at -20 ° C. Seed viability test was carried out in media: that are modified of Knudson'C (KC), Vacin and Went (VWs), Hyponex (HS) and Knudson C + micro elements (KCA). The results show that D. discolor seeds can be stored for up to 60 years, D. macrophyllum seeds can last up to 20 years, while D. crumenatum seeds lose viability after 9 years of storage. After nine years, the highest percentage of germination achieved by D. discolor was around 52.17%. The best medium for D. macrophyllum and D. crumenatum seed germination was KC. While D. discolor seeds germinated well on HS media.