In vitro propagation of Asplenium aethiopicum through tissue culture

Abstract

Poor viability of spores and germination and slow growth in conventional vegetative propagation are major constraints in the in vitro culture of Asplenium aethiopicum, an important medicinal fern. In the present study, a procedure for in vitro propagation of this endangered fern through croziers has been established. Maximum sporophytes efficiency was obtained on KC media supplemented with growth regulators such as 0.5mg/l NAA & 0.5mg/l 2,4-D with 2 sucrose under photoperiod condition. Maximum frequency (33.2%) of callus formation and germination of sporophyte was observed on KC medium with growth regulators.