Uji sitotoksisitas mikrofiber PMMA dan PMMA-Silika wetspinning pada kultur sel primer L-929 sebagai aplikasi penguat jembatan gigi direkCytotoxicity test of PMMA and PMMA-Silica wet spinning microfibers in L-929 primary cell culture as a direct dental bridge reinforcement application

Abstract

Introduction: Direct dental bridge consists of a fiber reinforcement component and a composite resin matrix component. The use of target cells for the cytotoxicity test of dental fiber materials is generally performed by experimental in-vitro tests to determine the clinical relevance of the test. This study was aimed to examine the cytotoxicity of PMMA and PMMA-silica wet spinning microfibers with different parameters on the primary cell culture (cell line) of L-929 fibroblasts. Methods: The research design was descriptive qualitative. Primary L-929 fibroblast cells were exposed to PMMA and PMMA-silica microfibers consecutively for 1, 4, and 7 days. Cytotoxicity test was performed using the MTT Assay. Parameters of PMMA and PMMA-silica microfibers used were concentration and flow rate, then divided into several research groups and named as follows: PMMA microfiber vertical system 250ml/hour with a concentration in %: 0.75(A); 1(B); 1.25(C); PMMA-silica microfiber vertical system with the speed of 200 ml/hour (D), 250 ml/hour (E), 300 ml/hour (F) and PMMA microfiber with rotation system 200 ml/ hour with a concentration in % 0.75(G );1(H), 1,25(I); PMMA-silica microfiber rotation system with concentrations of 200ml/hour (J), 250ml/hour (K), and 300 ml/hour (L). Results: In-vitro test of the L-929 cell picture showed no primary fibroblast cells that died. The cell line growth curve of each microfiber parameter shows that the cells can proliferate during the incubation period and show a positive trend of cell growth. Conclusions: PMMA and MMAsilica wet spinning microfibers did not show any toxicity to the growth of the L-929 fibroblast cell line, so they have potential as reinforcement applications for direct dental bridges.