On-chip microfluidics for proteomic analysis by electrospray ionization/mass spectrometry

Abstract

Describes the development of a silicon integrated demonstrator coupled to an ElectroSpray Ionization source in view of protein analysis by Mass Spectrometry (ESI/MS). The design and the making of basic components: (i) a novel thermal valveless micropump, (ii) a Solid Phase Extraction (SPE) column and (iii) a hollow cantilever nanoemitter constitute the steps of the authors' ongoing technological program which will be achieved by their gathering within a single chip. At present a manifold prototype at a 10 /spl mu/m scale has been fabricated and is currently being tested.