[A modification of the immunoenzyme method of determining class A immunoglobulins].

Abstract

The authors suggest a variant of enzyme immunoassay for measuring IgA in the blood and other biologic fluids. This method is based on the use of polystyrene balls, made in the USSR, as the solid phase; the balls are placed in biochemical tubes with a conic bottom. The sensitivity of the method is approximately 40 times superior to the widely known radial diffusion in gel. Use of polystyrene balls presensitized with antispecies antibodies helps cut down the time of the assay. The method is recommended for clinical laboratories that are intended for unsophisticated investigations.