Effect of High Fat and High Sugar Diet on Mouse Liver Redox Status

Abstract

This research is to find out if a high fat high sugar diet could affect the health of mice based on the level of energy products (ATP, ADP, AMP, NAD, NADH, NADP, NADPH, GSH, and GSSG) in the liver. In this research, I use HPLC to collect data for the level of each of the nine energy products. I used the PCA extraction method to extract liver tissues. In addition, I also used Lowry protein assay for the PCA extracts to compare to the results from HPLC.