STRUCTURE OF BRONCHIAL ARTERIES IN RATS WITH TRAUMATIC BRAIN INJURY AND SYSTEMIC INFLAMMATION

Abstract

Purpose of the study: to present a histochemical and morphometric assessment of the state of the blood vessels of the bronchial tree and lung parenchyma of rats in experimental traumatic brain injury (TBI). and systemic inflammation caused by the administration of pyrogenal. Materials and methods. The experiment was carried out on mature male Wistar rats (200-250 g). Potentially painful interventions in the ongoing experiments and euthanasia were performed under combined injection anesthesia. The model of the falling weight modified by us for rats was used to reproduce TBI. To initiate systemic inflammation (SI), the animals were intramuscularly injected with pyrogenal solution. The study included the following groups: intact rats (n=12); after applying TBI (n=12)-TBI; triple administration (1, 3, 4 days) of pyrogenal without TBI (n=16)-SV; triple administration of pyrogenal (1, 3, 4 days) after TBI (n=12)-TBI+SV. On days 4 and 6 from the beginning of the experiment, under general anesthesia, the animals were dissected, their lungs were removed, after which histological sections were made. Morphological and functional changes in the blood vessels of the bronchial tree and lung parenchyma were analyzed in experimental traumatic brain injury (TBI) and systemic inflammation in rats. It has been established that in injured rats, the administration of pyrogenal has a significant effect on the structure of the bronchial blood vessels of the lungs, while the application of only TBI does not initiate such changes. At the same time, a significant change in the morphometric characteristics of blood vessels is detected, a spasm of arterioles is determined with a decrease in the diameter of the lumen on days 4 and 6 of the study, and an accumulation of segmented leukocytes, macrophages and other bronchial secretion cells in the perivascular space of the vessels is noted. Thus, the results of the study show that with the comorbidity of TBI and SI in the lungs, structural changes occur in the elastic framework of the distal bronchial arteries, which manifests itself changes in the structure of the elastic membranes of these blood vessels.