Spectrophotometry Setup for Solute Concentration Determination

Abstract

Human body has a high transmittance coefficient for near infrared light. Therefore, dyes with notable absorbance peaks in the near infrared range have a variety of applications in diagnostics. Whereas one of the main liver functions is blood purification, the rate of removal of dye from the blood is direct index of liver functionality. Indocyanine green is used to measure liver function with plasma disappearance rate parameter. Dynamic measurement of dye concentration in human blood allows to calculate plasma disappearance rate. Thereby, our setup is intended to be applied for liver function assessment. In water solution indocyanine green has absorption peak on 790 nm. Absorption analysis method of solution concentration determination is considered. We present spectrophotometry setup for measurement of the dye concentration in solution.