Structure and function of aconitase enzyme in TCA by estimating optical rotation of glucose and determining the relationship between cell density and absorbance

S. Raza, A. Ameen
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Abstract

Enzyme aconitase have a great value in TCA path, this enzyme use to convert pyruvate and acetyl co A in to citrate and cis aconitase ( a six carbon molecule). This study was designed to find the tertiary structure of aconitase with and without ligand by using RSBC. The TCA cycle start with the pyruvate that is end product of glycolysis cycle. This study also focused on the optical rotation of glucose molecule before its breakdown start naturally through glycolysis and absorbance / transmittance of viable cells would be estimated .
通过估计葡萄糖旋光度和测定细胞密度与吸光度的关系来研究TCA中乌头酸酶的结构和功能
乌头酸酶在TCA途径中具有重要的应用价值,它将丙酮酸和乙酰辅酶a转化为柠檬酸和顺式乌头酸酶(一种六碳分子)。本研究旨在用RSBC法确定有配体和无配体的乌头酸酶的三级结构。三羧酸循环从丙酮酸开始,丙酮酸是糖酵解循环的最终产物。本研究还关注葡萄糖分子通过糖酵解自然分解前的旋光性,并估计活细胞的吸光度/透射率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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