EVALUASI TITER ANTIBODI PASCA VAKSINASI Septicaemia epizootica PADA SAPI BALI DI KOTA KUPANG

JURNAL KAJIAN VETERINER Pub Date : 2020-06-19 DOI:10.35508/jkv.v8i1.2292

Mario H.Cantona, M. U. Sanam, Tri Utami, T. Tophianong, Antin Y. N. Widi

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Abstract

Controlling Septicemia epizooticae (SE) through vaccination program has been undertaken in Kupang City. However, numbers of fatal cases are still being reported. The purpose of this study is to measure the antibody titer of Bali cattle after SE vaccination, and to determine the effect of age and sex on antibody titers. The 50 serum samples of SE vaccinated Bali cattle were taken from Alak Sub-district (26 samples) and Maulafa Sub-district (24 samples). The selection of sub-districts in Kupang City was taken in a simple random manner. Those serum samples were examined using the indirect enzyme-linked immunosorbent assay (ELISA) method. Antibody titers against SE is declared to be protective when the antibody titer is above 88 ELISA Unit (EU). Results indicated that average value of cattle antibody titer after the SE vaccination was able to trigger a protective antibody response (> 70 EU), meanwhile ONE WAY ANOVA analysis results showed that there is no significant effect (P> 0.05) of cattle age towards antibody titers. In the same way, the paired t test results did not indicate a difference in the value of antibody titers against the sex of the Bali cattle.

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在库邦市的巴厘岛奶牛中，对术后抗体的鉴定

库邦市通过疫苗接种计划控制家畜败血症。然而，仍在报告死亡病例的数量。本研究的目的是测定巴厘牛接种SE后的抗体效价，并确定年龄和性别对抗体效价的影响。分别从Alak街道(26份)和Maulafa街道(24份)采集了50份接种了SE疫苗的巴厘牛血清样本。古邦市街道的选择采用简单随机的方式。采用间接酶联免疫吸附试验(ELISA)检测血清样品。当抗体滴度高于88 ELISA Unit (EU)时，对SE具有保护作用。结果表明，牛接种SE后的抗体滴度平均值能够触发保护性抗体反应(> 70 EU)，而单因素方差分析结果显示，牛年龄对抗体滴度没有显著影响(P> 0.05)。以同样的方式，配对t检验结果没有表明抗体滴度值对巴厘牛性别的差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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