Optimization of Xylanase Activity in the Presence of Salts of Different Metals for the Pulping of Indigenous Non-Woody Raw Materials (Gossypium Arborium)

Abstract

This study was conducted to optimize the process of degradation of agricultural wastes using xylanase by pulping. The cotton stalk was treated with enzyme and sugar with different concentration was obtained. The substrate concentration, incubation time, cofactors and activators on xylanase were investigated in the pulping and bleaching process. The study presents results that at pH 6.0 with time span (3, 6 and 9 hours) of incubation produced 56 mg/ml, 75 mg/ml and 112.5 mg/ml sugar respectively. While at pH 7.0 with time duration (3, 6 and 9 hours) of incubation 64 mg/ml, 75 mg/ml and 150 mg/ml of sugar was produced and at pH 8.0 with time duration (3, 6 and 9 hours) of incubation produced 64 mg/ml, 220 mg/ml and 225 mg/ml sugar. There was no significant change in production of sugar at pH 9.0, where during the time period (3, 6 and 9 hours) of incubation 30 mg/ml, 37 mg/ml and 45 mg/ml of sugar was produced respectively. While metal salts are good activators, for the time period of 3, 6 and 9 hours of incubation 56 mg/ml, 64 mg/ml and 90 mg/ml of sugar was produced. This work provides an efficient method for organic waste disposal using an environment-friendly approach with a wide industrial application for welfare of society.