Culturable Cellulolytic Bacteria From Mangrove With Anadara Granosa Cultivation In Sukal, West Bangka

Abstract

Sukal, West Bangka district became one of the centers of Anadara sp culture. Not yet known the effect of shellfish cultivation on the ability of decomposition in mangroves. Cellulolytic bacteria are part of degradation in mangroves. Cellulolytic bacteria have cellulose degradation ability and it made carbohydrates are easier about digested for livestock. This research aims to obtained cellulolytic bacteria in soil, decayed wood and leaf from mangrove with Anadara sp culture in Sukal, West Bangka. The research was conducted from March until June 2018. Isolation was done using agar media with 1% Carboxymethyl Cellulose (CMC). An isolate of bacteria grew in agar with 1% CMC and given the Lugol and Congo Red at 72 hours to test cellulose degradation ability. Isolates from Sukal Mangrove that result the largest cellulose degradation had their species identification using 16S rRNA gene sequenced and microbact analysis. The results of biochemical tests using Mikrobact for bacterial isolates cellulolytic show selected lead to species Bacillus megaterium, Citrobacter freundii, and Vibrio alginoliticus. DNA sequences of isolate bacteria showed close kinship with Pseudomonas psychotolerans. Keywords–Phylogeny, Cellulolytic Bacteria, Mangrove, Anadara sp culture