The toxicity of Meistera chinensis rhizome fraction by shrimp larvae with the BSLT method

Abstract

Meistera chinensis is a species that belong to the Zingiberacea family. In Southeast Sulawesi, a population of Meistera chinensis was found in the Abuki forest of Konawe Regency. Empirically, its consumed by the community as a cooking spice and body fitness. The results of previous research revealed that Meistera chinensis fruit is efficacious as an antioxidant, anticancer, antimicrobial and antifungal. Research on the rhizome has never been done. This study aims to determine the potential for toxicity and the LC50 value of Meistera chinensis rhizome using the BSLT method. The rhizomes were extracted with 96% ethanol and concentrated using a rotary evaporator. Then fractionation was carried out with methanol and ethyl acetate as solvents. The concentration of the rhizome fraction was 7.81 ppm, 15.63 ppm, 32.25 ppm, 62.5 ppm, 125 ppm, 250 ppm, 500 ppm and 1000 ppm. Positive control used of 1.4 mg potassium bichromate and negative control is DMSO. Toxicity was determined based on the mortality of shrimp larvae and calculated by determining the LC50 value. The results showed that the LC50 values ​​for the methanol, ethyl acetate and positive control fractions of Meistera chinensis rhizome were 252.88 mg/L, 57.17 mg/L, and 6.22 mg/L, respectively. Based on these results, it can be concluded that the Meistera chinensis rhizome fraction has a toxic effect on shrimp larvae (≤1,000 mg/L).