Large scale extraction of alpha-lactalbumin and beta-lactoglobulin from bovine whey by precipitation with polyethylene glycol and partitioning in aqueous two-phase systems.

A Ortín, J A Cebrian, G Johansson
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引用次数: 6

Abstract

The milk proteins alpha-lactalbumin and beta-lactoglobulin have been isolated from bovine whey by fractional precipitation with polyethylene glycol (PEG) and hydrophobic partitioning in aqueous PEG-hydroxypropylstarch two-phase systems using PEG-bound palmitate as hydrophobic ligand. The possible use of this combination for large scale purification of these whey proteins is discussed.

聚乙二醇沉淀法和双水相分离法从牛乳清中大规模提取α -乳清蛋白和β -乳球蛋白。
采用聚乙二醇(PEG)分步沉淀法,以聚乙二醇结合棕榈酸酯为疏水配体,在聚乙二醇-羟丙基淀粉两相体系中疏水分配,从牛乳清中分离出乳蛋白α -乳清蛋白和β -乳球蛋白。讨论了该组合用于大规模纯化乳清蛋白的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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