Other sea star Igkappa gene cloning assay in E. coli with new parameters

Abstract

The plasmid vector pET-28b(+) named “Young” was produced according a work of 2014.1 This construct is designed to allow the expression of a 13.6 kDA protein with a C-terminal 6histag. It is supposed to be an anti-HRP (Horse-radish peroxydase protein). This protein was not expressed in first E. coli: we attempt to explain this phenomenon.