THE EFFECT OF COFFEE ON THE SPERMATOZOA MOTILITY OF MALE WISTAR RATS (Rattus norvegicus) EXPOSED TO ELECTRIC CIGARETTE SMOKE

DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Pub Date : 2021-05-31 DOI:10.14710/DMJ.V10I3.29987

Erika Shinta Avianty, P. Dewi, A. N. Setyawati, Arufiadi Anityo Mochtar

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Abstract

Background: Man infertility was caused by decrease of spermatozoa quality. E-cigarette smoke which contained chemicals as nicotine could produce free radicals which decreased spermatozoa motility. Coffee was contained of caffeine and chlorogenic acid functioned as antioxidant and increase spermatozoa motility. Aim: To prove the coffee giving effect to spermatozoa motility of male wistar rats that have been exposed to e-cigarette smoke. Methods: The sample were 28 male wistar rats (Rattus norvegicus), no anatomical abnormalities, 200-300 grams, and 8-9 weeks. The sample was randomly divided into 4 groups: no treatment, the rats which were exposed to e-cigarette smoke, the rats which were given 3ml robusta coffee solution contained of 180mg coffee powder and exposed to e-cigarette smoke, the rats which were given 3ml robusta coffee solution contained of 360mg coffee powder and exposed to e-cigarette smoke. The coffees solution was given through a gastric swab. The e-cigarette liquid doze was 3,5ml with 18mg/ml nicotine for each group. After four weeks, the rat’s sperm sample was taken, preparation was made, and spermatozoa motility was examined through microscope. The statistical test exerted Saphiro-Wilks test and non-parametrical test from Kruskal-Wallis. Results: The mean of spermatozoa motility percentage in progressive, non-progressive, and negative control group immotility = (53,81±6,507), (30,00±5,444), (16,66±3,333); positive control = (0,48±1,259), (17,14±18,604), (82,38±19,787); first treatment = (10,00±4,304), (32,85±14,065), (57,14±17,259); second treatment = (11,42±1,779), (26,19±8,033), (62,38±8,760). Kruskal-Wallis test showed a significant difference in progressive motility and immotility (p=0,000). Conclusion: The coffee solution could give an effect on the spermatozoa motility of male wistar rats exposed to electric cigarette smoke. Keywords: Infertility, e-cigarette, nicotine, coffee, motility

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咖啡对接触电子烟烟雾的雄性褐家鼠精子活力的影响

背景:男性不育是由精子质量下降引起的。含有尼古丁等化学物质的电子烟烟雾会产生自由基，从而降低精子的活力。咖啡中含有咖啡因和绿原酸，具有抗氧化和增强精子活力的作用。目的:探讨咖啡对接触电子烟的雄性wistar大鼠精子活力的影响。方法:取雄性褐家鼠28只，解剖无异常，200 ~ 300克，8 ~ 9周龄。将样本随机分为4组:未处理组、暴露于电子烟烟雾的大鼠、给予3ml含180mg咖啡粉的罗布斯塔咖啡溶液暴露于电子烟烟雾的大鼠、给予3ml含360mg咖啡粉的罗布斯塔咖啡溶液暴露于电子烟烟雾的大鼠。咖啡溶液是通过胃拭子注射的。电子烟液量为3.5 ml，每组尼古丁含量为18mg/ml。4周后取大鼠精子标本，进行制备，显微镜下观察精子活力。统计检验采用saphiroo - wilks检验和Kruskal-Wallis的非参数检验。结果:进行性、非进行性、阴性对照组精子活力百分率均值分别为(53,81±6,507)、(30,000±5,444)、(16,66±3,333);阳性对照=(0,48±1,259)，(17,14±18,604)，(82,38±19,787);首次治疗=(10,00±4,304)，(32,85±14,065)，(57,14±17,259);第二次治疗=(11,42±1,779)，(26,19±8,033)，(62,38±8,760)。Kruskal-Wallis检验显示进行性运动和不运动有显著差异(p= 0000)。结论:咖啡溶液对接触电子烟烟雾的雄性wistar大鼠精子活力有影响。关键词:不孕症，电子烟，尼古丁，咖啡，运动

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DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)

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