In Vitro Evaluation of the Influence of Extraction Solvents on Antioxidant and Anti-Inflammatory Potentials of Dried Leaf of Bryophyllum pinnatum Lam.

Abstract

Summary This study investigated the effect of different extraction solvents on the phenolic contents (TPC), antioxidant activities, and anti-inflammatory potentials of dried-leaf extracts of Bryophyllum pinnatum Lam. The analyses were performed spectrophotometrically, using a Folin-Ciocalteau reagent for the estimation of the total phenolic contents. The ferric reducing power (FRP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays were carried out to assess the antioxidant activities of aqueous, ethanolic, ethyl acetate, and n-hexane extracts of the plant, whereas the stabilization of heat-treated albumin and inhibition of protease action were performed to evaluate the anti-inflammatory potentials of the extracts. The results obtained indicate that the solvent nature significantly influenced the amount of TPC, antioxidant and anti-inflammatory potentials of the extracts. The aqueous extract had the highest TPC. The FRP was equally high in the aqueous and ethanol extracts. The ethanolic extract had the highest radical scavenging activity and anti-inflammatory potentials. The results show that water and ethanol could be suitable solvents for the extraction of bioactive compounds from the dried leaf of B. pinnatum vis-à-vis antioxidant and anti-inflammatory potentials.