The modern methods of reproduction physiology of horses

A. Tkachev, O. Tkacheva, B. Gutyj
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Abstract

The concept of development of horse breeding in Ukraine until 2020 provides for an increase in the number of horses through the intensive use of modern methods of reproduction biotechnology. However, the imperfection of these methods hinders their widespread use in practice. The aim of the work was to draw attention to the most important problems of the physiology of horse reproduction in Ukraine and to show ways to solve them that have already been proposed by domestic and foreign researchers. The development strategy of the physiology of horse reproduction in order to increase its effectiveness should take into account the least studied aspects that were discussed above. The article shows that taking into account the influence of micromycetes, the absolute number of colony forming units of E. coli in semen of stallions; immuno-and cytogenetic features; new methods of sanitary preparation of horses for sperm and insemination; the effect of permissible levels of feed mycotoxins on physiological functions improves the efficiency of equine reproduction physiology methods. However, we first discovered new physiological features of the effect of erythrocyte antigens of blood groups of horses of Ukrainian selection on the indicators of their native sperm. In the presence of ad/bcm and dg/cgm alleles of the blood group D system in stallions, sperm motility is on average less than 5 points; alleles ad/cgm, ad/d, ad/de, ad/dk, bcm/d, bcm/de, bcm/dg, bcm/dk, cegm/cgm, cegm/d, cegm/dg, cegm/dk, cgm/ceg, cgm/cgm, cgm/dg, cgm/dk, de/cgm, de/dk, dg/di, dk/d, dk/de, dk/dk is accompanied by sperm motility from 5 to 7 points; alleles bcm/cgm, dg/dk, de/d, cgm/d, cgm/de sperm motility is observed more than 7 points. The results obtained allowed us to develop for practice ways to increase the efficiency of sperm cryopreservation by immunogenetic parameters. In addition, open physiological correlations can increase the fertility of mares during mating.
马的繁殖生理学的现代方法
乌克兰到2020年的马育种发展概念规定,通过密集使用现代繁殖生物技术方法来增加马的数量。然而,这些方法的不完善阻碍了它们在实践中的广泛应用。这项工作的目的是引起人们对乌克兰马繁殖生理学中最重要问题的关注,并展示国内外研究人员已经提出的解决这些问题的方法。为了提高马生殖生理学的有效性,其发展策略应该考虑到上面讨论的研究最少的方面。本文表明,考虑到微菌的影响,种马精液中大肠杆菌菌落形成单位的绝对数量;免疫和细胞遗传学特征;马的精子和授精卫生准备的新方法饲料真菌毒素允许水平对生理功能的影响提高了马生殖生理学方法的效率。然而,我们首先发现了乌克兰选种马血型红细胞抗原对其原生精子指标影响的新生理特征。在D血型系统ad/bcm和dg/cgm等位基因存在的情况下,种马精子活力平均小于5分;等位基因ad/cgm, ad/d, ad/de, ad/dk, bcm/d, bcm/dg, bcm/dk, cegm/cgm, cegm/d, cegm/dg, cegm/dk, cgm/ceg, cgm/cgm, cgm/dg, cgm/dk, de/cgm, de/dk, dg/di, dk/d, dk/de, dk/dk伴随精子活力5 ~ 7分;等位基因bcm/cgm、dg/dk、de/d、cgm/d、cgm/de的精子活力均大于7点。所得结果使我们能够开发出通过免疫遗传学参数提高精子冷冻保存效率的实践方法。此外,开放的生理关联可以提高交配时母马的生育能力。
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