LAGE3 is a potential therapeutic target and prognostic factor in breast cancer: database mining for LAGE family members in human malignancies

Abstract

Breast cancer is the top cancer in women worldwide. Human L antigen (LAGE) family, are a cluster of genes that located on Chromosome X. To date, 4 members of LAGE family named LAGE1, LAGE2A, LAGE2B and LAGE3 have been reported, but their roles contributing to tumorigenesis and prognosis of breast cancer are still not clear. In the present study, we studied the expression of LAGE transcripts in an array of tumor types, compared with the normal controls using ONCOMINE database, and the corresponding expression files in a variety of cancer cell lines through CCLE analysis, along with the protein expression in normal breast tissues and breast cancer specimens from the Human Protein Atlas (HPA). Gene Expression-Based Outcome for Breast Cancer Online (GOBO) analysis was used to examine the mRNA expression in breast cancer patients with different intrinsic PAM50 subtypes. Based on Kaplan-Meier plotter, the prognostic value in breast cancer patients, was evaluated. It was found that, LAGE3 was distinctly high expressed at mRNA and protein levels in breast cancer patients when compared with normal tissues among all LAGE members. In survival analysis, low expression of LAGE3 was correlated to better survival in breast cancer patients, which revealed that expression of LAGE3 is a predictor of poor prognosis in breast cancer. In conclusion, LAGE3 is a distinct biomarker and essential prognostic factor for breast cancer.