PARTICULARITY OF INTRODUCING OF NEW BREEDED GRAPEVINES INTO CULTURE IN VITRO

Abstract

The paper presents the results of the intro-duction into the culture in vitro of grapevine varieties Ilya, Karmakod, Preobragenue. To develop chemotherapy, the introduction of salicylic acid, the antibiotic Cefotaxim and the growth regulator Melafen was studied. The slow development of the meristem dur-ing the first 43–55 days of cultivation was observed. The studied drugs slightly stimu-lated this process. Melafen's positive influ-ence was marked. Different reactions of the studied varieties to the conditions of cultiva-tion in vitro both at the stage of introduction and at the subsequent stage of micro-propagation have been revealed. The Karmakode variety has shown a high adap-tive capability to in vitro conditions and no response to the drugs used. 363 shoots were formed. In the variants with salicylic acid (0.77 mg/l) and Cefotaxim (100 mg/l), there was a suppression of regenerative ability. In the variety Ilya on the nutrient medium with Melafen, the cut of shoots increased com-pared to control by 2.6 times. When re-entering the culture of variety Preobragenie better micropropagation was marked. 73 shoots were cut. The predominant effect of apical meristems in comparison with chemo-therapy has been revealed. Active develop-ment of the meristem was with additional cultivation them in a rotating state on a roll-er-type device.