Koyun alveoler makrofajlarında Mannheimia haemolytica'ya yanıt olarak bağışıklık ile ilişkili genlerin in vitro mRNA ekspresyon dinamikleri

Abstract

Alveolar macrophages (AMs) respond to these infections as the first line of defense and trigger the lung's immune response. Knowing the expression dynamics of key immune-related genes in alveolar macrophages against M. haemolytica challenge will help deepen the understanding of disease immunopathogenesis. For this purpose, first time an in vitro experimental model of infection was used to investigate the expression pattern of Interleukin-1β, IL-6, IL-8, IL-10, and NF-κB in a dose and time-dependent manner. A total of 95% pure alveolar macrophages culture plates were obtained from bronchoalveolar lavage (BAL) fluid and treated with three doses (T1: 1800, T2: 2700, T3: 5400 CFU) of M. haemolytica inoculum, while keeping one untreated as a control. Then, total RNA was extracted, and cDNA was synthesized. The real-time quantification data indicated that the expression of IL-1β, IL-6, IL-8, IL-10, and NF-κB genes were significantly changed as compared to control. Our study revealed that the exposure to M. haemolytica stimulates the immune response in the sheep alveolar macrophages in a dose and time-dependent manner.