To compare the different scaffolds according to porosity's properties

Abstract

The main purpose of this study is to investigate the increase in osteoblast cells cultured on three different porosity scaffolds in vivo environment. Hence, Collagen + Poly-l-lactic acid (PLLA), Collagen +HA (30 C°), Collagen + HA (37 C°) were studied. During the operation, 20 ml bone marrow was taken for any of patient in sterile conditions. Mesenchymal stem cells were differentiated to osteoblast cells. After the ALP and morphological evaluations were assessed, osteoblast cells were planted on 3 different scaffolds and SEM observations were evaluated. At twenty-fifth day, konfleunt osteoblast cells were observed. Osteoblast cells were watched on Collagen + HA (30 C°) scaffold appropriately. According to our knowledge, in various bone defects, cell cultures on suitable scaffolds are contribute to clinical studies.