Role of Light Emitting Diode (LED) Fluorescence Microscopy in the Diagnosis of Smear Negative Pulmonary Tuberculosis.

Z. Khatun, C. Roy, T. Sultana, Q. Rahman, A. Ahmed
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Abstract

Light emitting diode (LED) fluorescence microscopy offers well described benefits compared with brightfield microscopy by Ziehl-Neelsen stained sputum, even which are smear negative. We evaluated the diagnostic performance of fluorescence microscopy, using novel light-emitting diode (LED) technology as an alternative to the brightfield microscopy. The objective of this study was the role of LED fluorescence microscopy in diagnosis of smear negative pulmonary tUberculosis. This is a prospective study consisted of 50 smear negative patients, who were clinically suspected cases of pulmonary tuberculosis. All samples were stained by both ZN stain and Auramine stain and as a gold standard all were cultured on Lowenstein-Jensen Media. On evaluation of all sputum samples were found negative by ZN method but by auramine stain 16%, 20%, 20% cases were found positive by conventional fluorescence microscopy (CFM), LED and culture respectively. LED fluorescence microscopy is more useful test to distinguish the smear negative cases. It also provide an effective guideline to make decisions regarding judicious use of antitubercular drug therapy. Keywords: Smear negative Sputum; LED; CFM; Culture; Pulmonary Tuberculosis DOI: http://dx.doi.org/10.3329/bjpath.v26i1.9126 BJPATH 2011; 26(1): 3-6
发光二极管荧光显微镜在涂片阴性肺结核诊断中的作用。
发光二极管(LED)荧光显微镜提供了良好的描述与明场显微镜的Ziehl-Neelsen染色痰,即使是涂片阴性。我们评估了荧光显微镜的诊断性能,使用新型发光二极管(LED)技术作为明场显微镜的替代品。本研究的目的是LED荧光显微镜在诊断涂片阴性肺结核中的作用。这是一项前瞻性研究,包括50例涂片阴性患者,临床怀疑为肺结核病例。所有样品均采用ZN染色和Auramine染色,并作为金标准在Lowenstein-Jensen培养基上培养。所有痰液经锌法检测均为阴性,而金胺染色法检测阳性率分别为16%、20%、20%,常规荧光显微镜、LED和培养检测阳性率分别为20%。LED荧光显微镜是鉴别涂片阴性病例更有用的方法。它也为明智地使用抗结核药物治疗提供了有效的指导。关键词:涂片阴性痰;领导;CFM;文化;肺结核DOI: http://dx.doi.org/10.3329/bjpath.v26i1.9126 BJPATH 2011;26 (1): 3 - 6
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