Phytochemicals potentiate interleukin-2 generated lymphokine-activated killer cell cytotoxicity against murine renal cell carcinoma.

Molecular biotherapy Pub Date : 1992-09-01

Y Wang, X J Qian, H R Hadley, B H Lau

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Abstract

The success of adoptive immunotherapy using recombinant interleukin-2 (rIL-2) and lymphokine-activated killer (LAK) cells in several cancers has been hampered by severe toxicity associated with high doses of rIL-2. Methods that reduce the dosage of rIL-2 without loss of clinical efficacy are needed. In this study we determined the in vitro effect of a phytochemical immune modulator, Astragalus membranaceus (AM), and two fractions isolated by high-performance liquid chromatography on the cytotoxicity of rIL-2-generated LAK cells against a murine renal cell carcinoma. Our results indicated a 10-fold potentiation of rIL-2-generated LAK cell cytotoxicity manifested by tumor cell lysis of 88% in the group with 100 U/ml of rIL-2 plus AM versus 86% in the group with 1,000 U/ml of rIL-2 alone. Potentiation was obtained with the purified fractions as well. A significantly reduced number of LAK cells was required to achieve the tumor cytotoxicity after LAK cell generation with rIL-2 plus the phytochemicals as compared with rIL-2 alone. Our data indicate that AM is an effective immune modulator, capable of potentiating in vitro the antitumor activity of rIL-2-generated LAK cells.

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植物化学物质增强白细胞介素-2产生的淋巴因子激活的杀伤细胞对小鼠肾细胞癌的细胞毒性。

利用重组白细胞介素-2 (il -2)和淋巴因子活化杀伤(LAK)细胞对几种癌症进行过继免疫治疗的成功受到高剂量il -2相关的严重毒性的阻碍。需要在不丧失临床疗效的情况下减少il -2剂量的方法。在本研究中，我们测定了植物化学免疫调节剂黄芪(Astragalus membrane aceus, AM)和高效液相色谱分离的两个组分对il -2生成的LAK细胞对小鼠肾细胞癌的细胞毒性的体外作用。我们的研究结果表明，il -2产生的LAK细胞毒性增强了10倍，在100 U/ml的il -2加AM组中，肿瘤细胞裂解率为88%，而在单独1000 U/ml的il -2组中，肿瘤细胞裂解率为86%。纯化后的部分也得到了增强作用。与单独使用rIL-2相比，使用rIL-2和植物化学物质生成LAK细胞后，实现肿瘤细胞毒性所需的LAK细胞数量显著减少。我们的数据表明AM是一种有效的免疫调节剂，能够增强il -2生成的LAK细胞的体外抗肿瘤活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular biotherapy

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