The feasibility of atomic force microscopy as a cytodetachment technique to quantify osteoblastic adhesion with implant surfaces

D. Gianoli, S. Kohles, N. Burnham, M. B. Clark, C. Brown, J. Kenealy
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引用次数: 3

Abstract

The preliminary data acquired for the roughness parameter (Ra) demonstrate that there is a clear difference between the 4 different surface preparations studied. Based on previous work it can be expected that once each sample has been seeded with cells, there will exist strong variations among the cellular activity on each disk surface. While it is not the goal of this project to quantify differences among the levels of cellular activity, it is believed that they can be inferred from differences in adhesive strength as well as visual differences among the images produced by the AFM. It is expected that differences in extracellular matrix production will be chiefly responsible for not only differences in cellular phenotypes among samples but also differences in adhesive strengths.
原子力显微镜作为细胞剥离技术量化成骨细胞与种植体表面粘附的可行性
获得的粗糙度参数Ra的初步数据表明,所研究的4种不同表面制备之间存在明显差异。根据以前的工作,可以预期,一旦每个样本都播种了细胞,每个磁盘表面的细胞活动之间将存在强烈的变化。虽然这个项目的目标不是量化细胞活动水平之间的差异,但人们相信,它们可以从粘附强度的差异以及AFM产生的图像之间的视觉差异中推断出来。预计细胞外基质生产的差异不仅是样品中细胞表型差异的主要原因,也是黏附强度差异的主要原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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