Dielectrophoresis, cell culture, and Electrical Impedance Spectroscopy Applied to Adherent Cells in a Single Biochip

Abstract

In this work, we present a device that performs the 4 following functions: fluidic handling of biological cells suspension, electrical manipulation of cells by dielectrophoresis, cell culture and electrical impedance detection of adherent cells. Microfabrication process of the device, a hybrid glass/metal/PDMS structure, is detailed. Dielectrophoretic manipulation, culture and cell detection have been performed in a single device using human hepatocellular liver carcinoma cell. Results of broadband (10-107 Hz) electrical impedance spectroscopy (EIS) measurement performed on human cancerous cells are exposed