Perbedaan Morfologi dan Fragmentasi DNA Sperma sebelum dan sesudah Kriopreservasi dengan Metode Slow Cooling di Klinik Aster RSUP Dr. Hasan Sadikin Bandung

Faizal Arif Caropeboka, Tono Djuwantono, Dian Tjahyadi, J. Effendi, A. D. Anwar, Amillia Siddiq
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Abstract

Abstrak Tujuan : Penelitian ini bertujuan untuk mengetahui perbedaan parameter fragmentasi DNA, morfologi sperma pasca proses pembekuan dengan metode slow cooling. Metode : Penelitian ini merupakan penelitian obervasional analitik dengan pendekatan pre-post design. Subjek penelitian adalah sperma dengan hasil analisis yang normal sesuai dengan standar WHO (n=25). Penelitian dilakukan di Klinik Aster RSUP Dr. Hasan Sadikin Bandung pada bulan Juli hingga Agustus 2017. Hasil : Setelah proses kriopreservasi, terdapat peningkatan fragmentasi DNA tiga kali lipat (nilai p<0,05) dan terdapat penurunan jumlah morfologi normal sebesar 50% (nilai p<0,05). Kesimpulan : Terdapat penurunan kualitas sperma pasca proses kriopreservasi dengan metode slow cooling. Kata kunci : Fragmentasi DNA, morfologi, slow cooling, spermatozoa Abstract Objective : Of this study was to compare sperm quality parameters including DNA fragmentation and morphology after cryopreservation with slow-cooling method. Method : This was an analytical observational study with pre and post design. Subjects were men whose sperm analysis met the WHO criteria of being normal (n=25). The study was conducted at the Aster Clinic of Dr. Hasan Sadikin General Hospital Bandung from July to August 2017. Results : After cryopreservation, there was a three fold increase of DNA fragmentation (with p value <0.05) and a decrease in morphology 50% (with p value <0.05). Conclusion: There is a decrease in sperm quality after cryopreservation with slow-cooling method. Key words : DNA fragmentation, morphology, slow cooling, spermatozoa
哈桑·萨德金·万隆分泌物诊所用缓慢蒸馏法观察精子和脱氧核糖核酸
抽象目的:这项研究的目的是用缓慢蒸馏的方法来确定脱氧核糖核酸(精子)在冷冻后的形态形态。方法:本研究是一种具有产前设计方法的obervational分析研究。根据世卫组织标准,检测结果正常的精子(n=25)。这项研究于2017年7月至8月在Hasan Sadikin万隆的Aster RSUP诊所进行。结果:经过低温处理,脱氧核糖核酸的脱氧核糖核酸的脱氧核糖核酸增长三倍(p< 0.05),正常的形态学数量减少了50% (p< 0.05)。结论:用慢炖的方法观察精子后质的质量下降。关键词:脱氧核糖核酸、形态学、慢抖、精子分析方法:这是一项具有预测和后设计的分析研究。研究对象是精于分析的普通人。该研究于2017年8月至8月由哈桑·萨德金综合医院(Hasan Sadikin General Hospital)策划。建议:在低温保存后,DNA片段有三种增量(p值<0.05)和50%形态分解(p值<0.05)。结论:低温保存后的精油中有一种脱粒,采用缓慢冷却的方法。关键字:DNA片段、形态学、缓慢冷却、精子
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