Cytotoxic Effects of Ceteareth-20 and Paraffinium Liquidum In Vitro

Journal of Toxicology and Risk Assessment Pub Date : 2022-03-31 DOI:10.23937/2572-4061.1510043

Kirkik Duygu, Hacimustafaoglu Fatih, Altunkanat Derya

{"title":"Cytotoxic Effects of Ceteareth-20 and Paraffinium Liquidum In Vitro","authors":"Kirkik Duygu, Hacimustafaoglu Fatih, Altunkanat Derya","doi":"10.23937/2572-4061.1510043","DOIUrl":null,"url":null,"abstract":"Aim: The aim of this study is to investigate the effects of ceteareth-20 and paraffinium liquidium on cell viability and cytotoxicity in human lymphocyte in vitro . Methods: We studied the cytotoxic and inhibitory effects of ceteareth-20 and paraffinium liquidum on cell proliferation using lactate dehydrogenase (LDH) assay and cell proliferation (WST-1) assay. Results: The cytotoxicity was enhanced when cells were treated with 1%, 5%, 25% and 50% paraffinium liquidium dilutions (p < 0.05). Moreover, cell number significantly reduced after 24 hours when they were treated with the same concentrations of paraffinium liqudium (p < 0.05). On the other hand, ceteareth-20 at concentrations at 1%, 5%, 25% and 50% showed no significant cytotoxic effect. These results showed that paraffinium liquidum dilutions have cytotoxic and proliferative effect otherwise ceteareth-20 dilutions have only proliferative effects on cultured human lymphocytes. Conclusion: It can be said that paraffinium liquidum and ceteareth-20 is harmful to use in beauty and cosmetic products. Future studies might allow alternative agents to be used instead of paraffinium liquidum and ceteareth-20.","PeriodicalId":174677,"journal":{"name":"Journal of Toxicology and Risk Assessment","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Toxicology and Risk Assessment","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.23937/2572-4061.1510043","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Aim: The aim of this study is to investigate the effects of ceteareth-20 and paraffinium liquidium on cell viability and cytotoxicity in human lymphocyte in vitro . Methods: We studied the cytotoxic and inhibitory effects of ceteareth-20 and paraffinium liquidum on cell proliferation using lactate dehydrogenase (LDH) assay and cell proliferation (WST-1) assay. Results: The cytotoxicity was enhanced when cells were treated with 1%, 5%, 25% and 50% paraffinium liquidium dilutions (p < 0.05). Moreover, cell number significantly reduced after 24 hours when they were treated with the same concentrations of paraffinium liqudium (p < 0.05). On the other hand, ceteareth-20 at concentrations at 1%, 5%, 25% and 50% showed no significant cytotoxic effect. These results showed that paraffinium liquidum dilutions have cytotoxic and proliferative effect otherwise ceteareth-20 dilutions have only proliferative effects on cultured human lymphocytes. Conclusion: It can be said that paraffinium liquidum and ceteareth-20 is harmful to use in beauty and cosmetic products. Future studies might allow alternative agents to be used instead of paraffinium liquidum and ceteareth-20.

查看原文本刊更多论文

石蜡-20和石蜡的体外细胞毒作用

目的:研究石蜡-20和石蜡液体对体外人淋巴细胞活力和细胞毒性的影响。方法:采用乳酸脱氢酶(LDH)法和细胞增殖法(WST-1)，研究茜草醚-20和石蜡液的细胞毒作用和对细胞增殖的抑制作用。结果:1%、5%、25%、50%石蜡液稀释后细胞毒性增强(p < 0.05)。同样浓度石蜡液处理24h后，细胞数量明显减少(p < 0.05)。另一方面，在1%、5%、25%和50%浓度下，乙酸乙酯-20没有明显的细胞毒性作用。结果表明，石蜡液稀释液对培养的人淋巴细胞具有细胞毒性和增殖作用，而乙酸乙酯-20稀释液对培养的人淋巴细胞只有增殖作用。结论:液体石蜡和石蜡-20在美容化妆品中使用是有害的。未来的研究可能会允许使用替代剂来代替石蜡和石蜡-20。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Toxicology and Risk Assessment

自引率

0.00%

发文量