Detection of biotin-streptavidin interaction using RF interdigitated capacitive cavity

Abstract

In this paper, biotin streptavidin interaction has been sensed at microwave frequencies with the help of designed sensor. It takes advantage of two pole filter topology where interdigitated capacitor (IDC) is used as a coupling zone between resonators for higher sensitivity. Due to permittivity change of biotin streptavidin combination, shift in resonant frequency arise whereas due to conductivity, magnitude of reflection coefficient changes. The frequency range of 10 GHz − 25 GHz (γ- dispersion region) is used as it is effective for label free analysis of biotin streptavidin. Due to homogeneous E-field between the fingers of IDC, placement position of biotin-streptavidin will not affect the sensitivity. Significant frequency shifts as high as 19 MHz, 45 MHz and 70 MHz are observed for concentrations as low as 10 ng/ml, 20 ng/ml and 30 ng/ml of biotin-streptavidin respectively. Simulation results suggest that proposed biosensor is sensitive even for concentration of 5 ng/ml of sample.