Specific biodetection of a murine spontaneous mammary carcinoma with labelled anti-human blood group-A monoclonal antibody

International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology Pub Date : 1992-07-01 DOI:10.1016/0883-2897(92)90154-Q

Alberto L. Horenstein , Amalia Koss , Lucas L. Colombo , Horacio M. Glait

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引用次数: 3

Abstract

The expression of cell surface antigens of the spontaneous transplantable M3-murine tumour was studied by means of the monoclonal antibody (MAb) B2C114 which recognizes the human blood group-A carbohydrate antigen. Following radioiodination the MAb retained their immunoreactivity and demonstrated a significantly higher in vitro binding with isolated M3-tumour cells as compared with a control antibody. B2C114 revealed 10⁶ antigenic sites per cell, with a constant affinity of 5.1 × 10⁹/M. Biodistribution studies showed that B2C114 discriminated between malignant tumour and mouse normal tissues. Radioimmunodetection of Balb/c mice bearing s.c. M3-tumour showed that tumour was specifically defined without substraction 1 day after injection of the radiolabelled antibody.

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标记抗人血a型单克隆抗体对小鼠自发性乳腺癌的特异性生物检测

用识别人血a型碳水化合物抗原的单克隆抗体(MAb) B2C114研究了自发性m3 -小鼠肿瘤细胞表面抗原的表达。放射碘化后，MAb保持了免疫反应性，与对照抗体相比，与分离的m3肿瘤细胞的体外结合明显更高。B2C114在每个细胞中显示106个抗原位点，具有5.1 × 109/M的恒定亲和力。生物分布研究表明，B2C114能够区分恶性肿瘤和小鼠正常组织。放射免疫检测显示，注射放射性标记抗体1天后，携带sc - m3肿瘤的Balb/c小鼠肿瘤特异性定义，无减法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology

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