Fabrication of Microbioreactors with an Optimized Structure Designed for High Density Culture of Hepatocyte

Abstract

In order to perform cell culture from soft tissue in good condition, i.e. maintaining high viability and keeping specific physiological function, scaffolds with microstructured surface mimicking in vivo environment is necessary. In this paper we proposed PDMS microbioreactors with high surface/volume ratio for hepatocyte culture. These PDMS microbioreactors were designed for cells to attach in high density (over 108 cells/cm3), and were characterized in terms of flow resistance, which is important for mass transport of nutrients and oxygen