Auxin regulation in the interaction between Rice stripe virus and rice.

Chinese Journal of Agricultural Biotechnology Pub Date : 2009-04-01 DOI:10.1017/S1479236209002502

Yang Jin-guang, Wang Wen-ting, Ding Xin-Lun, Guo Li-juan, Fang Zhen-Xing, Xie Liyan, Lin Qi-ying, Wu Zujian, Xie Lian-hui

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引用次数: 2

Abstract

The expression of YUCAA1 gene and the amount of endogenous IAA in rice(Oryza sativa subsp,japonica)plants and rice suspension cells infected by Rice stripe virus(RSV)were investigated by Real-time RT-PCR and high performance liquid chromatography,respectively.And the results showed that the expression of YUCAA1 gene and the amount of endogenous IAA increased at various times(16,32,48 and 64 h)after post-infection by RSV in rice suspension cells.In rice plants infected by RSV,the expression of YUCAA1 gene and the amount of endogenous IAA increased at 4～8 d after post-infection as comparison with that of healthy rice plants,and decreased at 12 and 16 d.These results indicated that RSV infection could regulate auxin biosynthesis in rice. Additionally,the expression of RSV CP increased 2.9 times in rice plants after it was treated with KPSC buffer to deplete the endogenous auxins,and decreased 45% after 30μmol/L IAA treatment.All of these results suggest that the auxin may play a role among RSV replication in rice plant.

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水稻条纹病毒与水稻互作中生长素的调控。

采用Real-time RT-PCR和高效液相色谱法分别检测了水稻(Oryza sativa subsp,japonica)植株和水稻悬浮细胞中YUCAA1基因的表达和内源IAA的含量。结果表明，水稻悬浮细胞感染RSV后，YUCAA1基因表达量和内源IAA量在不同时间(16、32、48和64 h)均呈上升趋势。在RSV感染水稻植株中，YUCAA1基因的表达量和内源IAA的表达量在感染后4~8 d较健康水稻有所增加，在感染后12 d和16 d有所下降，说明RSV感染可以调节水稻生长素的生物合成。用KPSC缓冲液处理后，水稻植株中RSV CP的表达量增加了2.9倍，而用30μmol/L IAA处理后，RSV CP的表达量下降了45%。这些结果提示生长素可能在水稻RSV复制过程中起一定作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Chinese Journal of Agricultural Biotechnology

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