Development and Application of CRISPR-Mediated Genetic Screening in Oncology

Abstract

Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool for gene editing. Moreover, it can also be applied to genetic screening to investigate various biological activities for its high efficiency and throughput. The common procedure of CRISPR-mediated genetic screening includes the identification of genes of interest, the construction of sgRNA library, the transduction of sgRNA library on expression vector into target hosts, and the following data analysis. CRISPR-mediated genetic screening would further develop, employing various Cas protein and regulating diverse life process. This review article summarizes the development of CRISPR, the common procedure of CRISPR-mediated genetic screening, and the future applications.