{"title":"Antitumor activity of some polysaccharides isolated from a Chinese mushroom, \"huangmo\", the fruiting body of Hohenbuehelia serotina.","authors":"Y Ma, T Mizuno, H Ito","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Polysaccharides were extracted from a Chinese mushroom, \"Huangmo\" with hot water (FI), 1% ammonium oxalate solution (FII), and 5% sodium hydroxide (FIII) and 20 more polysaccharides were isolated by ethanol precipitation, ion-exchange chromatography, gel filtration, and affinity chromatography. Of the six polysaccharides obtained, FI0, FA-1, FA-2, FII-2, FIII-1-b, and FIII-2-b were selected on the basis of the results of the antitumor activity test (the Sarcoma 180/mice, i.p. method). Six fractions which showed relatively high activities were examined: FI0, a mixture of galactose-containing heteroglycan and beta-D-glucan; FA-1 and -2, beta-D-glucan with galactose-containing hetero-glycan and protein; FII-2, beta-D-glucan; and FIII-1-b and FIII-2-b, (1----6)-beta-D-glucosyl branched (1----3)-beta-D-glucan.</p>","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"55 11","pages":"2701-10"},"PeriodicalIF":0.0000,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural and biological chemistry","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Polysaccharides were extracted from a Chinese mushroom, "Huangmo" with hot water (FI), 1% ammonium oxalate solution (FII), and 5% sodium hydroxide (FIII) and 20 more polysaccharides were isolated by ethanol precipitation, ion-exchange chromatography, gel filtration, and affinity chromatography. Of the six polysaccharides obtained, FI0, FA-1, FA-2, FII-2, FIII-1-b, and FIII-2-b were selected on the basis of the results of the antitumor activity test (the Sarcoma 180/mice, i.p. method). Six fractions which showed relatively high activities were examined: FI0, a mixture of galactose-containing heteroglycan and beta-D-glucan; FA-1 and -2, beta-D-glucan with galactose-containing hetero-glycan and protein; FII-2, beta-D-glucan; and FIII-1-b and FIII-2-b, (1----6)-beta-D-glucosyl branched (1----3)-beta-D-glucan.
采用热水(FI)、1%草酸铵溶液(FII)和5%氢氧化钠溶液(FIII)分别提取黄磨菇多糖，并通过乙醇沉淀、离子交换层析、凝胶过滤和亲和层析等方法分离出20余个多糖。根据抗肿瘤活性试验(Sarcoma 180/小鼠，i.p.法)的结果，从得到的6种多糖中选择FI0、FA-1、FA-2、FII-2、FIII-1-b和FIII-2-b。研究了六个具有较高活性的组分:FI0，含半乳糖的杂聚糖和β - d -葡聚糖的混合物;FA-1和-2，β - d -葡聚糖与含半乳糖的异聚糖和蛋白质;FII-2 beta-D-glucan;以及FIII-1-b和FIII-2-b， (1----6)- β - d -葡萄糖基支链(1----3)- β - d -葡聚糖。
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