Rapid analysis and quantification of proteins in high throughput using PAIA assays

Abstract

The PAIA technology provides a novel modular platform for the quantification of proteins. It combines the advantages of bead-based immunoassays with sensitive fluorescence detection in a simple, automation-friendly workflow. The no-wash assays can be processed in one hour per plate and are measured on fluorescence plate readers or microscopes. The amount of sample required is very small, typically 2 10 μL. Thus, the assays are particularly suitable for high throughput antibody and protein quantification in samples like cell culture supernatants. The modular nature of the patent pending PAIA technique offers the possibility to address a large number of analytes.