Development of cell-sheet handling tool for measurement of cell sheet adhesion force

Abstract

We have demonstrated hybrid micro mechanical systems (HMMS) using muscle cells. When constructing cells to HMMS, there are some forces between adhesive interface and cells, dur to contraction of muscle cells must be considered to design. When these forces are larger than adhesion force of cells, cells detach from substrates and system is not functional. Measuring the adhesion force of cells could prevent from the breakage of the system. In this paper, we developed cell adhesion force measurement system “90 degree detachment examination” which defined by “International Organization for Standardization (ISO)”. To attempt 90 degree detachment examination, we noticed that to use cell sheet which cultured confluent on two-dimensional surface. And to use cell sheet, we develop four types of cell sheet handling tools. As a result, we measured maximum adhesion strength of cells about 10 N/mm.