A Temperature Resistant Extracts Prepared from the Perinereis aibuhitensis and Its Antioxidative Characterization

Abstract

The Perinereis aibuhitensis (P. aibuhitensis), a traditional Chinese medicine, has a function of delaying senilityaccording to “Compendium of Materia Medica”. It is considered a useful animal model in antioxidant activity tests. The P. aibuhitensis has already been utilized as biochemical responses to specific pollutants and the food supply for various birds. In the present work, the extracts from P. aibuhitensis were prepared by the traditional decocting method and the biocompatibility was characterized by hemolysis and MTT tests. The materials showed no hemolytic for the hemolysis rate (lower than 0.2 %) while exhibited moderate cytotoxic effects on HUVECs (Human Umbilical Vein Endothelial Cells). In extracellular antioxidant activity against O2¯ and ·OH, the extracts from P. aibuhitensis could eliminate O2¯ and ·OH efficiently. In cellular ROS level tests, the ROS level was reduced effectively after treated with P. aibuhitensis extracts. The extracts had a potential prospect as a biological antioxidant to reduce redundant reactive oxygen species damage and great significance for humans health.