A new neutralization method for influenza virus in cell culture.

The Kitasato archives of experimental medicine Pub Date : 1992-12-01

F Taguchi, O Hashimoto, T Matsuzaki, S M Lee

引用次数: 0

Abstract

A novel neutralization method (ELISA-NT) has been developed for simple detection of neutralizing antibodies to influenza virus in which type specific soluble (S) CF antigen produced by unneutralized virus was measured by anti-S immune (probe) serum and enzyme-labelled Clq. Neutralizing antibody activity was determined within 48 h and expressed as a new term, NT% of the quantity of neutralized virus. A good correlation was found between NT% determined by the ELISA-NT method and NT titer by the conventional method in 82 human serum samples (r = 0.941 for type A and r = 0.875 for type B of influenza virus, p < 0.01).

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细胞培养中和流感病毒的新方法。

建立了一种新的中和方法(ELISA-NT)，用于简单检测流感病毒中和抗体，该方法采用抗S免疫(探针)血清和酶标记Clq检测未中和病毒产生的型特异性可溶性(S) CF抗原。在48 h内测定中和抗体活性，并用新项NT%表示中和病毒的数量。82份人血清中ELISA-NT法测定的NT%与常规方法测定的NT滴度有较好的相关性(A型和B型分别r = 0.941和0.875,p < 0.01)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Kitasato archives of experimental medicine

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