Evaluation of desferal as a bifunctional chelating agent for labeling antibodies with Zr-89

Abstract

Zirconium-desferal was prepared and analysed by TLC, NMR and u.v.-spectroscopy. The stoichiometry of the complex was found to be 1:1. Chelation of desferal, coupled to resin, with ⁸⁸Zr appeared to be fast and almost quantitative in various buffer systems in a broad pH-range (4–7). A high in vitro stability of the zirconium-desferal complex was observed; less than 0.2% zirconium was lost within 24 h in plasma-solutions.