Sex and age dependence of rat kidney sialidase.

Enzyme Pub Date : 1992-01-01 DOI:10.1159/000468809
L Cohen-Forterre, J André, M Sternberg
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引用次数: 2

Abstract

Rat kidney sialidase levels have been reported to be markedly altered in pathological states such as diabetes. This was associated with a modification of sialic acid levels. Therefore, it was interesting to study the variations of kidney sialidase and sialyltransferase activities and sialic acid content according to sex and age. This was carried out from birth to 210 days of age. The substrates used were sialyl alpha(2-3)[3H]-lactitol for sialidase activity, asialofetuin and [14C]-CMPNeu5Ac for sialyltransferase activity. In males sialidase activity increased until 32 days then slightly declined. In females, the activity increased and leveled off at 135 days of age. Higher sialidase activity was observed in females than in males from 56 days of age. Gonadectomy had no effect on this activity. In both sexes, sialyltransferase activity decreased markedly with age. This activity was higher in females than in males, whereas sialic acid levels varied only moderately with age and were slightly higher in females.

大鼠肾唾液酸酯酶的性别和年龄依赖性。
据报道,大鼠肾唾液酸酶水平在糖尿病等病理状态下显着改变。这与唾液酸水平的改变有关。因此,研究肾脏唾液酸酶、唾液酸转移酶活性和唾液酸含量在性别和年龄上的变化是很有意义的。这项研究从出生到210日龄进行。所使用的底物为唾液酸酶活性的唾液酸α (2-3)[3H]-乳酸醇,唾液酸蛋白和唾液酸转移酶活性的唾液酸转移酶[14C]-CMPNeu5Ac。雄虫唾液苷酶活性上升至32 d后略有下降。在雌性中,这种活动在135日龄时增加并趋于平稳。从56日龄开始,雌性的唾液酸酶活性高于雄性。性腺切除术对这种活性没有影响。在两性中,唾液基转移酶活性随着年龄的增长而明显下降。这种活性在女性中高于男性,而唾液酸水平仅随年龄适度变化,在女性中略高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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