STUDI IN VITRO EKSTRAK DAUN TEMBAKAU (Nicotiana tabacum) DAN DAUN BINAHONG HIJAU (Anredera cordifolia steenis) SEBAGAI INSEKTISIDA ALAMI UNTUK MYIASIS PADA RUMINANSIA

Teguh Dwi Putra, Nur Taufiq, Hamzah Nata Siswara, Lia Nur Aini, Khoirul Huda
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Abstract

The purpose of this study was to determine the effectiveness of the combination of tobacco leaf extract (Nicotiana tabacum) and green binahong leaf (Anredera cordifolia steenis) against myiasis. The study used the in vitro method for testing, while the leaf extract was obtained using the immersion method. This research was conducted using a completely randomized design (CRD) with a one-way pattern (One Way ANNOVA) with 6 treatments and 4 replications. The test uses fresh beef samples that have been rotten for 6 days at room temperature. The fresh weight of each meat sample was 100 grams. The treatments were P0 (control: only water), P1 (100% tobacco), P2 (100% green binahong), P3 (50% tobacco: 50% green binahong), P4 (70% tobacco: 30 green binahong) and P5 (30% tobacco: 70% green binahong). The results observed in the study included the time (seconds) required for larvae to come out of the meat after spraying on each treatment, the death of myiasis larvae at 5, 10, 15, and 25 minutes. The results showed that treatment P4 got the best results. compared with other treatments with a significant difference at the level of p <0.05. Key words: Tobacco leaves, Binahong leaves, Myiasis, Animal health.
本研究的目的是确定烟叶提取物(Nicotiana tabacum)与绿比纳红叶(Anredera cordifolia steenis)联合使用对蝇蛆病的防治效果。本研究采用离体法进行试验,叶提取物采用浸渍法获得。本研究采用完全随机设计(CRD),采用单向模式(One Way ANNOVA),共6个处理,4个重复。该试验使用在室温下已腐烂6天的新鲜牛肉样品。每个肉类样品的新鲜重量为100克。处理分别为P0(对照:纯水)、P1(100%烟草)、P2(100%绿滨拿洪)、P3(50%烟草:50%绿滨拿洪)、P4(70%烟草:30%绿滨拿洪)和P5(30%烟草:70%绿滨拿洪)。研究中观察到的结果包括每次喷洒后幼虫从肉中出来所需的时间(秒),幼虫在5、10、15和25分钟死亡。结果表明,P4治疗效果最好。与其他处理比较在p <0.05水平上有显著差异。关键词:烟叶,槟榔叶,蝇蛆病,动物保健
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