Microsatellite Variability of Two Populations of Clarias gariepinus (Siluriformes, Clariidae) in Nigeria

M. Awodiran, F. O. Adeniran, R. Akinwale, A. A. Akinwande
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引用次数: 2

Abstract

Abstract The study evaluated the genetic signatures of the fishes from the two populations and compared the pattern of differentiation of the two populations with a view to separating the species from the different populations into possible sub-species. Forty (40) specimens were collected from River Niger (Lokoja) and Asejire Resevoir. The DNA of the twenty (20) specimens from each population extracted from the muscle tissue using phenol-chloroform extraction (PCE) method was subjected to microsatellite DNA analysis. Seven (7) microsatellite markers (Cga01, Cga02, Cga03, Cga05, Cga06, Cga09 and Cga10) were used in the analysis. Microsatellite DNA analysis of the two populations revealed significant differentiation between the two populations as shown by the high values of heterozygosity, low level of inbreeding and non-conformance to Hardy-Weinberg’s equilibrium. It is concluded from the study that microsatellite analysis showed a high potentiality for separation of the populations.
尼日利亚两居群gariepinclarias (siluriformia, Clariidae)的微卫星变异
摘要本研究评估了两个种群的遗传特征,并比较了两个种群的分化模式,以期从不同种群中划分出可能的亚种。从尼日尔河(Lokoja)和Asejire水库采集标本40(40)份。采用苯酚-氯仿萃取(PCE)法从肌肉组织中提取各种群20个样本,进行微卫星DNA分析。采用7个微卫星标记(Cga01、Cga02、Cga03、Cga05、Cga06、Cga09和Cga10)进行分析。对两个群体的微卫星DNA分析表明,两个群体的杂合度较高,近交水平较低,不符合Hardy-Weinberg平衡。结果表明,微卫星分析具有较高的群体分离潜力。
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