Protective role of Withania somnifera root extract on lipid peroxidation of Erythrocytes

M. A. Abu Zaid, M. Chauhan, Swarndeep Chauhan
{"title":"Protective role of Withania somnifera root extract on lipid peroxidation of Erythrocytes","authors":"M. A. Abu Zaid, M. Chauhan, Swarndeep Chauhan","doi":"10.51129/ujpah-2022-33-2(1)","DOIUrl":null,"url":null,"abstract":"Withania Somnifera (Ashwagandha) is an important medicinal plant that has been used in Ayurvedic and indigenous medicine. It is also known as Indian ginseng. It exerts antioxidant, anti-inflammatory and immune modulatory activities. In the present study, we studied the free radical scavenging activity of Withania Somnifera root extract. The radical scavenging effect of the extract was studied by its effect on hydroxy radicals generated by Fe-ascorbate-H2O2 system. Our results showed that Withania Somnifera root extract (1-10,000 µg/ ml) resulted in a significant dose dependent increase in percent inhibition of hydroxylation. Further, we studied the in vitro effect of Withania Somnifera root extract on Lipid peroxidation in terms of MDA level of human erythrocytes. Our results showed a significant dose dependent decrease in lipid peroxidation at a concentration of (1-1000 µg/ ml) as evident by decrease in level of MDA. Maximum effect was observed at 100-1000 µg/ ml. Withania Somnifera root extract thus can act as a potential antioxidant containing compound and protects erythrocytes from oxidative damage by either directly scavenging the free radical, sparing it or activating other enzymatic processes. At higher concentration (10,000 µg/ ml), Withania Somnifera extract increased the oxidative stress as evident from erythrocyte MDA level which became significantly more than the maximum effect shown at 1000 µg/ ml of extract.","PeriodicalId":432227,"journal":{"name":"Universities' Journal of Phytochemistry and Ayurvedic Heights","volume":"16 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Universities' Journal of Phytochemistry and Ayurvedic Heights","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.51129/ujpah-2022-33-2(1)","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Withania Somnifera (Ashwagandha) is an important medicinal plant that has been used in Ayurvedic and indigenous medicine. It is also known as Indian ginseng. It exerts antioxidant, anti-inflammatory and immune modulatory activities. In the present study, we studied the free radical scavenging activity of Withania Somnifera root extract. The radical scavenging effect of the extract was studied by its effect on hydroxy radicals generated by Fe-ascorbate-H2O2 system. Our results showed that Withania Somnifera root extract (1-10,000 µg/ ml) resulted in a significant dose dependent increase in percent inhibition of hydroxylation. Further, we studied the in vitro effect of Withania Somnifera root extract on Lipid peroxidation in terms of MDA level of human erythrocytes. Our results showed a significant dose dependent decrease in lipid peroxidation at a concentration of (1-1000 µg/ ml) as evident by decrease in level of MDA. Maximum effect was observed at 100-1000 µg/ ml. Withania Somnifera root extract thus can act as a potential antioxidant containing compound and protects erythrocytes from oxidative damage by either directly scavenging the free radical, sparing it or activating other enzymatic processes. At higher concentration (10,000 µg/ ml), Withania Somnifera extract increased the oxidative stress as evident from erythrocyte MDA level which became significantly more than the maximum effect shown at 1000 µg/ ml of extract.
苦参根提取物对红细胞脂质过氧化的保护作用
Withania Somnifera (Ashwagandha)是一种重要的药用植物,已被用于阿育吠陀和土著医学。它也被称为印度人参。它具有抗氧化、抗炎和免疫调节活性。在本研究中,我们研究了苦参根提取物的自由基清除活性。通过对铁-抗坏血酸- h2o2体系生成的羟基自由基的清除作用,研究了提取物的自由基清除作用。结果表明,1-10,000µg/ ml的Withania Somnifera根提取物对羟基化抑制的百分比呈剂量依赖性增加。此外,我们还从体外红细胞MDA水平的角度研究了苦参根提取物对脂质过氧化的影响。我们的研究结果显示,在浓度为(1-1000µg/ ml)时,脂质过氧化的降低具有显著的剂量依赖性,这可以通过MDA水平的降低来证明。因此,Withania Somnifera根提取物可以作为一种潜在的含有抗氧化剂的化合物,通过直接清除自由基、保留自由基或激活其他酶促过程来保护红细胞免受氧化损伤。在较高浓度(10,000 μ g/ ml)下,从红细胞MDA水平可以看出,Withania Somnifera提取物增加了氧化应激,显著高于1000 μ g/ ml提取物的最大效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信