Development and Evaluation of the Integrated In Situ Analyzer for Gene-"IISA-Gene" for Microbiology in Extreme Environments

Abstract

In this study an integrated in situ analyzer system for gene analysis (IISA-Gene) is developed to perform gene analysis of microorganisms inhabiting in extreme environments such as deep-sea or deep subsurface. Microfabricated chips made of PDMS and glass is employed to achieve a totally miniaturized and integrated system. Bacterial cell lysis, DNA purification, PCR amplification and amplified fragment detection can be performed with IISA-Gene system including miniaturized pumping and optical detection device. The IISA-Gene developed in this study was evaluated and operated in real deep-sea environment