Disinvasive efficacy of chlorine-based preparations of domestic production for eggs of nematodes of the species Aonchotheca bovis parasitizing in sheep

Abstract

The paper presents the results of experimental researches on the determination of the disinvasion efficiency of chlorinated chemical preparations of domestic production of “Brovades-plus”, “Bi-dez” and “Dezsans” of NPF “Brovafarma” (Ukraine). The conducted researches on the basis of the parasitology laboratory found that the studied disinfectants possess disinvasive properties in respect of non-invasive test culture of eggs of nematodes of the species Aonchotheca bovis (son Capillaria bovis) López-Neyra, 1947, isolated from gonads of females of worms. The dissimilar stability of eggs of capillaries to the effect of the tested agents in vitro is proved. The most effective disinfectant in the case of eggs of capillaries of the species A. bovis was the preparation “Dezsan”. The high level of its disinvasive efficacy (DE – 91.14–100.0%) was registered at the application of the preparation in 1.0–2.0% of the exposure concentration for 10–60 minutes. The chemicals “Bi-dez” and “Brovadez-plus” proved to be less effective in the relatively non-invasive test culture of A. bovis eggs. The high level of efficiency (DE –92.41–100.00%) of the “Bi-dez” was achieved with its use at concentrations of 1.5% (exposure of 30 and 60 min) and 2.0% (exposure of 10–60 minutes) The “Brovadez plus” preparation in vitro proved to be the least effective means of non-invasive test culture of nematode eggs of the species A. bovis, since a high level of disinvasive efficacy (DE – 100.00%) was achieved only with the use of 2.0% solution per exposure 10–60 min. It was established that the experimentally tested chlorine-based means of domestic production resulted in the death of eggs in the culture in due to the destructive effect of the components of the preparations on the shell, the caps of eggs of the capillaries, as well as on the germ in the middle of the egg. In experimental cultures treated with the means, the destruction of the shell of the egg in the location of the caps, the exit of morulae outside, the cessation of development or shrinkage of the embryo and deformation of the egg shell were recorded.