Daya antibakteri fraksi etil asetat daun kemangi (Ocimum basilicum) terhadap Streptococcus mutans ATCC 25175 pada clear retainer secara in vitroAntibacterial potential of the ethyl acetate fraction of basil (Ocimum basilicum) leaves on Streptococcus mutans ATCC 25175 on a clear retainer

Abstract

Introduction: Orthodontic appliances can affect the oral cavity ecosystem by increasing the number of Streptococcus mutans bacteria. Clear retainer is an orthodontic appliance that requires sterilisation prior to usage. The basil plant (Ocimum basilicum) was developed as an alternative natural sterilisation material. This study was aimed to analyse the antibacterial potential by examining the inhibitory zone, minimum inhibitory concentration (MIC), minimum killing concentration (MKC), and count the number of Streptococcus mutans bacteria on clear retainers that have been sterilised with ethyl acetate fraction of basil leaves and chlorhexidine. Methods: This research was an experimental laboratory that used the 5% basil ethyl acetate fraction and 2% chlorhexidine as control. The population and sample were one ose of Streptococcus mutans ATCC 25175. Examination of the inhibitory zone and the number of colonies was performed on agar media with cultured bacteria after being incubated at 37°C for 48 hours. Microdilution testing was performed using the microdilution method using a 96 microplate incubated at 37°C for 48 hours. Measurement of MIC and MKC was conducted using a spectrophotometer on an ELISA reader. The results of the study were analysed with the t-test. Results: There was an inhibitory zone found in the group of basil with a concentration of 5% and the control group (2% chlorhexidine). MIC and MBC of basil leave at 3125 ppm and 6250 ppm, while chlorhexidine at 3,125 ppm and 6,250 ppm, respectively. Statistical t-test results showed no significant difference in the decreasing number of Streptococcus mutans colonies after sterilisation with basil leaves and chlorhexidine. Conclusions: The ethyl acetate fraction of basil has antibacterial potential on Streptococcus mutans, as seen by the presence of an inhibitory zone during the MIC and MKC examination and decreasing number of bacterial colonies on agar media.