Kinetic measurement of T4 following column chromatography.

Abstract

A kinetic measurement of the eluates from the column purification of T4 is described. The choice of cation exchange chromatography to reduce interfering X-ray contrast substances, and the influence of protein in the reaction have been evaluated. Data for precision, inter and intra assay reproducibility, detection limit and normal values are reported. Using sample volumes of 0.5 ml and an analysis time of one minute, the proposed method compares favourably with published methods.