Improvement of Hemagglutination on Plastic Microfluidic Chip

Abstract

Hemagglutination is a specific form of agglutination that involves red blood cells (RBCs). It has a limitation in view of emergency and point-of-care diagnoses (POCD) due to the requirement of large scale equipment and long time to result. In this study, a plastic microfluidic chip (P-μFC) included dried antibody-embedded was introduced. The hemagglutination reaction was enhanced by increasing amount of antibody dissolved in a reaction chamber. Within 2 minutes, the blood agglutination reaction was evaluated quantitatively by image processing. Moreover, a protein stabilizers of sugars were employed to increase the solubility of dried antibody so that the reaction with dried antibody was equivalent to which with fresh antibody.