Screening and Identification of New Types of Exopolysaccharides-Producing Lactic Acid in the Inner Mongolia Dairy Products

Acta Universitatis Cibiniensis. Series E: Food Technology Pub Date : 2019-12-01 DOI:10.2478/aucft-2019-0010

Wenfei Shangguan, He Chen, Yilin Li, Zihua Wang, Hongxing Guo, J. Meng

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Abstract

Abstract Exopolysaccharides (EPS) is a type of polysaccharide produced by lactic acid bacteria (LAB) that can be directly used in foods to make the products more excellent. Therefore, batch studies were performed to explore the effect of different LAB on the production of EPS and antioxidant activity. Five strains with high EPS yield and antioxidant activity were screened out from 66 strains isolated from Tibetan dairy products. The results show that EPS produntion of the five strains (B55, B62, B30, 7830 and K2) were 110.66, 145.48, 132.78, 122.11 and 111.72 mg·L−1, respectively, and they have a higher DPPH free radical scavenging activity (56.29, 66.43, 62.94, 68.71, 61.87%). Five LAB strains were identified and classified based on screening, purification and 16S rDNA sequences. Molecular characterization based on partial sequence 16S rDNA homology confirmed the initial identification as Lactobacillus fermentum (B55, B62), Lactobacillus plantarum (7830), Pediococcus acidilactici (B30) and Lactobacillus helveticus (K2).

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内蒙古乳制品中新型产外多糖乳酸的筛选与鉴定

摘要外多糖(Exopolysaccharides, EPS)是由乳酸菌(LAB)产生的一种多糖，可直接用于食品中，使产品更加优良。因此，我们进行了批量研究，以探索不同LAB对EPS产生和抗氧化活性的影响。从西藏乳制品分离的66株菌株中筛选出5株EPS产量高、抗氧化活性高的菌株。结果表明，5株菌株(B55、B62、B30、7830和K2)的EPS产量分别为110.66、145.48、132.78、122.11和111.72 mg·L−1，对DPPH自由基的清除能力分别为56.29、66.43、62.94、68.71和61.87%。通过筛选、纯化和16S rDNA序列对5株LAB菌株进行鉴定和分类。基于16S rDNA部分序列同源性的分子鉴定初步鉴定为发酵乳杆菌(Lactobacillus fermentum, B55, B62)、植物乳杆菌(Lactobacillus plantarum, 7830)、酸性乳酸球球菌(Pediococcus acidilactii, B30)和helveticus乳杆菌(Lactobacillus helveticus, K2)。

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Acta Universitatis Cibiniensis. Series E: Food Technology

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